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#1 05-06-2024 11:08:34
- marciabrady
- Membre
Cell line immortalizing
Cell line immortalization is a crucial tool in research, enabling indefinite cell proliferation. Immortalized cell lines, like HEK293 and NIH3T3, offer time and energy savings compared to frequent primary cell isolation. Immortalization can occur naturally or be induced by factors such as SV40 T antigen, human TERT, and HPV E6-E7. Replication-deficient lentiviruses, efficient gene delivery tools, are commonly used for immortalization, especially in challenging primary cells. Creative Biogene provides lentiviral particles containing genes like SV40 T, human TERT, and HPV-16 E6/E7 for establishing immortalized cell lines.
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#2 01-09-2024 12:16:04
- wolfeh
- Membre
Re: Cell line immortalizing
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#3 01-09-2024 12:17:18
- wolfeh
- Membre
Re: Cell line immortalizing
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#4 30-10-2024 21:59:10
- wolfeh
- Membre
Re: Cell line immortalizing
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#5 30-10-2024 22:00:25
- wolfeh
- Membre
Re: Cell line immortalizing
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